免疫荧光病理诊断不典型膜性肾病的分子标志物研究

张鹏，张兴旺，朱静，崔波

（榆林市第一医院，陕西 榆林，719000）

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摘要：

目的 分析免疫荧光病理检查中Igs、C1q、C3 和FRA 诊断AMN 的应用价值。方法 选择我院经肾脏穿刺活检病理确诊 的30 例AMN 患者，30 例IMN 患者和30 例SMN 患者，观察分析三组患者的免疫荧光病理检查结果。结果 AMN 患者的肾小 球钉突形成和肾小管间质萎缩率高于IMN 及SMN 患者（P<0.05），肾小球硬化、炎性细胞浸润及肾小管炎性细胞浸润和纤维化改变率与IMN 及SMN 患者比较，无显著差异（P>0.05）。AMN 患者的IgA、IgM、C1q 和FRA 阳性沉积率高于IMN 和SMN 患 者（P<0.05），IgG 和C3 阳性沉积率与IMN 和SMN 患者比较，无显著差异（P>0.05）。AMN 患者的系膜细胞和系膜基质增生率 高于IMN 和SMN 患者（P<0.05），电子致密物沉积率与IMN 和SMN 患者比较，无显著差异（P>0.05）。结论 免疫荧光病理检 查Igs、C1q、C3 和FRA 对鉴别AMN 有重要应用价值。

关键词：免疫球蛋白分子；纤维蛋白相关抗原；不典型膜性肾病（AMN）

中图分类号：R692文献标志码：A文章编号：2096-1413(2017)35-0125-02

Molecular biomarkers study of Atypical membranous nephropathy by immunofluorescence pathological diagnosis
ZHANG Peng, ZHANG Xing-wang, ZHU Jing, CUI Bo


(the First Hospital of Yulin, Yulin 719000, China)

ABSTRACT: Objective To study the values of Igs, C1q, C3 and FRA by immunofluorescence pathological examination for AMN. Methods Thirty patients of AMN, 30 patients with IMN and 30 patients with SMN confirmed by pathological diagnosis of renal biopsy in our hospital were selected, and immunofluorescence pathological diagnosis results among the three groups were analyzed. Results The glomerular nail formation rate and kidney tubules interstitial atrophy rate in the AMN patients were higher than those of the IMN and SMN patients (P<0.05), the glomerular sclerosis, inflammatory cell infiltration rate and kidney tubules inflammatory cell infiltration and fibrosis changes rate were not significant with those of the IMN and SMN patients (P>0.05). The positive deposition rate of IgA, IgM, C1q and FRA in the AMN patients were higher than those of the IMN and SMN patients (P<0.05), the positive deposition rate of IgG and C3 were not significant with those of the IMN and SMN patients (P>0.05). The mesangial cell and mesangial matrix proliferation rate in AMN patients was higher than that in the IMN and SMN patients (P<0.05), the electron dense materials proliferation rate was not not significant with that of the IMN and SMN patients (P>0.05). Conclusion Igs, C1q, C3 and FRA immunofluorescence pathological diagnosis have important application effects in the identification of AMN.
KEYWORDS: immunoglobulin molecules; fibrin related antigen; atypical membranous nephropathy (AMN)

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