大剂量糖皮质激素对骨髓间充质干细胞成骨分化的 MiroRNA的调控作用

赵光辉，李辉，王志远

（西安市红会医院，陕西 西安，710054）

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摘要：

目的 探究不同浓度糖皮质激素对骨髓间充质干细胞（BMSC）成骨分化的MicroRNA 的调控作用。方法 利用10-8 mmol/L 及10-10 mmol/L 两组浓度地塞米松对BMSC 进行诱导，对比研究诱导7、14 d 时骨钙蛋白、骨桥蛋白及碱性磷酸酶的浓度变 化。结果 诱导7 d 时，两组的骨桥蛋白浓度均呈现升高趋势，且10-10 组骨桥蛋白浓度明显高于10-8 组（P<0.05）。诱导14 d 时，
10-8 组骨钙蛋白及骨桥蛋白的浓度降低，而10-10 组骨钙蛋白及骨桥蛋白的浓度升高，且10-10 组骨钙蛋白的浓度及骨桥蛋白 明显高于10-8 组（P<0.05）。诱导14 d 时，10-8 组碱性磷酸酶浓度升高，10-10 组碱性磷酸酶浓度降低，10-10 组明显低于10-8 组（P<0.05）。结论 高浓度的糖皮质激素诱导MicroRNA 调控BMSC 向脂肪细胞分化，抑制其向骨细胞分化。

关键词：地塞米松；骨髓间充质干细胞；MicroRNA

中图分类号：R681文献标志码：A文章编号：2096-1413(2017)34-0011-02

Regulatory role of high doses of glucocorticoids on MiroRNA in osteogenic differentiation of bone mesenchymal stem cells
ZHAO Guang-hui, LI Hui, WANG Zhi-yuan
(Xi``an Honghui Hospital, Xi``an 710054, China)

ABSTRACT: Objective To explore the regulation of different doses of glucocorticoids on MiroRNA in osteogenic differentiation of bone mesenchymal stem cells. Methods BMSC were induced by 10 -8 mmol/L and 10 -10 mmol/L of dexamethasone in two groups, and the concentration changes of osteocalcin, osteopontin and alkaline phosphatase at induced 7 and 14 days were compared. Results At induced 7 days, the concentrations of osteopontin in the two groups increased, and that in the 10-10 group was higher than the 10-8 group (P<0.05). At induced 14 days, the concentrations of osteocalcin and osteopontin in the 10-8 group decreased, and those in the 10-10 group increased, and those of the 10-10 group higher than the 10-8 group (P<0.05). At induced 14 days, the concentration of alkaline phosphatase in the 10-8 group increased, that in the 10-10 group decreased, and that in the 10-10 group was lower than the 10-8 group (P<0.05). Conclusion High concentration of glucocorticoids induced MicroRNA to regulate the differentiation of BMSC into adipocytes, and inhibit the differentiation of osteoblasts into adipocytes.
KEYWORDS: dexamethasone; bone mesenchymal stem cells; MicroRNA

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